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Structured Review

Proteintech anti tcf3
Anti Tcf3, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+tcf3/E2A+Antibody/pm41750997-114-45-48
Average 93 stars, based on 16 article reviews
anti tcf3 - by Bioz Stars, 2026-10
93/100 stars

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Related Articles

Activity Assay:

Article Title: LncRNA H19 inhibited dermal papilla cell senescence process through miR-29a by targeting Wnt/β-catenin signaling pathway.
Article Snippet: 1 Key Laboratory of Tumor Immunology and Microenvironmental Regulation, Guilin Medical University, Guilin, Guangxi 541004, P. R. China 2 Department of Neurosurgery, Second Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541100, P. R. China 3 Department of Dermatology, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541001, P. R. China 4 Tissue Engineering Laboratory, First Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong 515000, P. R. China 5 Department of Histology and Embryology, Shantou University Medical College, No. 22, Xinling Road, Shantou, Guangdong 515041, P. R. China 6 Institute of Basic Medicine, Guilin Medical University, Guilin, Guangxi 541004, P. R. China Abstract Androgenetic alopecia is a common type of hair loss disease.. As the most promising seeder for cell-based therapy, dermal papilla cells are prone to undergo premature senescence during passaging in vitro.. Our previous studies revealed high expression of lncRNA H19 in early-passage dermal papilla cells and the maintenance of hair follicle-inducing ability upon prolonged culture.

Immunofluorescence:

Article Title: LncRNA H19 inhibited dermal papilla cell senescence process through miR-29a by targeting Wnt/β-catenin signaling pathway.
Article Snippet: 1 Key Laboratory of Tumor Immunology and Microenvironmental Regulation, Guilin Medical University, Guilin, Guangxi 541004, P. R. China 2 Department of Neurosurgery, Second Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541100, P. R. China 3 Department of Dermatology, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541001, P. R. China 4 Tissue Engineering Laboratory, First Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong 515000, P. R. China 5 Department of Histology and Embryology, Shantou University Medical College, No. 22, Xinling Road, Shantou, Guangdong 515041, P. R. China 6 Institute of Basic Medicine, Guilin Medical University, Guilin, Guangxi 541004, P. R. China Abstract Androgenetic alopecia is a common type of hair loss disease.. As the most promising seeder for cell-based therapy, dermal papilla cells are prone to undergo premature senescence during passaging in vitro.. Our previous studies revealed high expression of lncRNA H19 in early-passage dermal papilla cells and the maintenance of hair follicle-inducing ability upon prolonged culture.

Staining:

Article Title: LncRNA H19 inhibited dermal papilla cell senescence process through miR-29a by targeting Wnt/β-catenin signaling pathway.
Article Snippet: 1 Key Laboratory of Tumor Immunology and Microenvironmental Regulation, Guilin Medical University, Guilin, Guangxi 541004, P. R. China 2 Department of Neurosurgery, Second Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541100, P. R. China 3 Department of Dermatology, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541001, P. R. China 4 Tissue Engineering Laboratory, First Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong 515000, P. R. China 5 Department of Histology and Embryology, Shantou University Medical College, No. 22, Xinling Road, Shantou, Guangdong 515041, P. R. China 6 Institute of Basic Medicine, Guilin Medical University, Guilin, Guangxi 541004, P. R. China Abstract Androgenetic alopecia is a common type of hair loss disease.. As the most promising seeder for cell-based therapy, dermal papilla cells are prone to undergo premature senescence during passaging in vitro.. Our previous studies revealed high expression of lncRNA H19 in early-passage dermal papilla cells and the maintenance of hair follicle-inducing ability upon prolonged culture.

Control:

Article Title: TRIM21 promotes colorectal cancer development through regulating DNA replication by TCF3/MCM2/5 axis
Article Snippet: Cells were lysed with Pierce® IP Lysis Buffer (Thermo Fisher Scientific, USA) and centrifuged. .. The supernatant was then divided and added with anti-TRIM21 (12108-1-AP, Proteintech, USA), anti-TCF3 (21242-1-AP, Proteintech, USA) or rabbit control IgG (AC005, ABclonal, China) antibody, followed by incubation overnight at 4 °C. .. The second day, each sample was added with Protein A/G-Agarose (Santa Cruz Biotechnology, USA) and incubated, followed by thorough washing and elution with loading buffer.

Article Title: TRIM21 promotes colorectal cancer development through regulating DNA replication by TCF3/MCM2/5 axis.
Article Snippet: Cells were lysed with Pierce® IP Lysis Buffer (Thermo Fisher Scientific, USA) and centrifuged. .. The supernatant was then divided and added with anti-TRIM21 (12108-1-AP, Proteintech, USA), anti-TCF3 (21242-1-AP, Proteintech, USA) or rabbit control IgG (AC005, ABclonal, China) antibody, followed by incubation overnight at 4 °C. .. The second day, each sample was added with Protein A/G-Agarose (Santa Cruz Biotechnology, USA) and incubated, followed by thorough washing and elution with loading buffer.

Incubation:

Article Title: TRIM21 promotes colorectal cancer development through regulating DNA replication by TCF3/MCM2/5 axis
Article Snippet: Cells were lysed with Pierce® IP Lysis Buffer (Thermo Fisher Scientific, USA) and centrifuged. .. The supernatant was then divided and added with anti-TRIM21 (12108-1-AP, Proteintech, USA), anti-TCF3 (21242-1-AP, Proteintech, USA) or rabbit control IgG (AC005, ABclonal, China) antibody, followed by incubation overnight at 4 °C. .. The second day, each sample was added with Protein A/G-Agarose (Santa Cruz Biotechnology, USA) and incubated, followed by thorough washing and elution with loading buffer.

Article Title: TRIM21 promotes colorectal cancer development through regulating DNA replication by TCF3/MCM2/5 axis.
Article Snippet: Cells were lysed with Pierce® IP Lysis Buffer (Thermo Fisher Scientific, USA) and centrifuged. .. The supernatant was then divided and added with anti-TRIM21 (12108-1-AP, Proteintech, USA), anti-TCF3 (21242-1-AP, Proteintech, USA) or rabbit control IgG (AC005, ABclonal, China) antibody, followed by incubation overnight at 4 °C. .. The second day, each sample was added with Protein A/G-Agarose (Santa Cruz Biotechnology, USA) and incubated, followed by thorough washing and elution with loading buffer.

Article Title: Wnt/β-Catenin Activation by iCRT3 Enhanced the Pluripotency of Bovine Expanded Pluripotent Stem Cells.
Article Snippet: .. After permeabilization and blocking with a solution containing 10% goat serum and 0.5% Triton X-100, the cells were incubated overnight at 4 ◦C with the following primary antibodies: anti-NANOG (bs0829R, BIOSS, China), anti-SOX2 (11064-1-AP, Proteintech, USA), anti-OCT4 (SC-5279, Santa Cruz Biotechnology, USA), anti-TCF1 (14464-1-AP, Proteintech), anti-TCF3 (21242-1- AP, Proteintech), anti-TCF4 (13838-1-AP, Proteintech), anti-LEF1 (abs158608, Absin, Chian), anti-β-catenin (51067-2-AP, Proteintech). .. Subsequently, the cells were incubated for 1 h at room temperature with species-appropriate secondary antibodies, including Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (A21206, Invitrogen), F(ab’)2-Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (A11017, Invitrogen).

Article Title: Wnt/β-Catenin Activation by iCRT3 Enhanced the Pluripotency of Bovine Expanded Pluripotent Stem Cells
Article Snippet: .. After permeabilization and blocking with a solution containing 10% goat serum and 0.5% Triton X-100, the cells were incubated overnight at 4 °C with the following primary antibodies: anti-NANOG (bs-0829R, BIOSS, China), anti-SOX2 (11064-1-AP, Proteintech, USA), anti-OCT4 (SC-5279, Santa Cruz Biotechnology, USA), anti-TCF1 (14464-1-AP, Proteintech), anti-TCF3 (21242-1-AP, Proteintech), anti-TCF4 (13838-1-AP, Proteintech), anti-LEF1 (abs158608, Absin, Chian), anti-β-catenin (51067-2-AP, Proteintech). .. Subsequently, the cells were incubated for 1 h at room temperature with species-appropriate secondary antibodies, including Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (A21206, Invitrogen), F(ab’)2-Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (A11017, Invitrogen).

Blocking Assay:

Article Title: Wnt/β-Catenin Activation by iCRT3 Enhanced the Pluripotency of Bovine Expanded Pluripotent Stem Cells.
Article Snippet: .. After permeabilization and blocking with a solution containing 10% goat serum and 0.5% Triton X-100, the cells were incubated overnight at 4 ◦C with the following primary antibodies: anti-NANOG (bs0829R, BIOSS, China), anti-SOX2 (11064-1-AP, Proteintech, USA), anti-OCT4 (SC-5279, Santa Cruz Biotechnology, USA), anti-TCF1 (14464-1-AP, Proteintech), anti-TCF3 (21242-1- AP, Proteintech), anti-TCF4 (13838-1-AP, Proteintech), anti-LEF1 (abs158608, Absin, Chian), anti-β-catenin (51067-2-AP, Proteintech). .. Subsequently, the cells were incubated for 1 h at room temperature with species-appropriate secondary antibodies, including Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (A21206, Invitrogen), F(ab’)2-Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (A11017, Invitrogen).

Article Title: Wnt/β-Catenin Activation by iCRT3 Enhanced the Pluripotency of Bovine Expanded Pluripotent Stem Cells
Article Snippet: .. After permeabilization and blocking with a solution containing 10% goat serum and 0.5% Triton X-100, the cells were incubated overnight at 4 °C with the following primary antibodies: anti-NANOG (bs-0829R, BIOSS, China), anti-SOX2 (11064-1-AP, Proteintech, USA), anti-OCT4 (SC-5279, Santa Cruz Biotechnology, USA), anti-TCF1 (14464-1-AP, Proteintech), anti-TCF3 (21242-1-AP, Proteintech), anti-TCF4 (13838-1-AP, Proteintech), anti-LEF1 (abs158608, Absin, Chian), anti-β-catenin (51067-2-AP, Proteintech). .. Subsequently, the cells were incubated for 1 h at room temperature with species-appropriate secondary antibodies, including Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (A21206, Invitrogen), F(ab’)2-Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (A11017, Invitrogen).



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